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  • 标题:Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport
  • 本地全文:下载
  • 作者:M R Block ; B S Glick ; C A Wilcox
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1988
  • 卷号:85
  • 期号:21
  • 页码:7852-7856
  • DOI:10.1073/pnas.85.21.7852
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us to purify the NEM-sensitive factor, which we term NSF. The NEM-sensitive factor is a tetramer of 76-kDa subunits, and appears to act catalytically, one tetramer leading to the metabolism of numerous transport vesicles.
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