期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2001
卷号：98
期号：5
页码：2577-2581
DOI：10.1073/pnas.041608298
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：In this study, we investigated the role of V14 natural killer T (NKT) cells in transplant immunity. The ability to reject allografts was not significantly different between wild-type (WT) and V14 NKT cell-deficient mice. However, in models in which tolerance was induced against cardiac allografts by blockade of lymphocyte function-associated antigen-1/intercellular adhesion molecule-1 or CD28/B7 interactions, long-term acceptance of the grafts was observed only in WT but not V14 NKT cell-deficient mice. Adoptive transfer with V14 NKT cells restored long-term acceptance of allografts in V14 NKT cell-deficient mice. The critical role of V14 NKT cells to mediate immunosuppression was also observed in vitro in mixed lymphocyte cultures in which lymphocyte function-associated antigen-1/intercellular adhesion molecule-1 or CD28/B7 interactions were blocked. Experiments using IL-4- or IFN-{gamma}-deficient mice suggested a critical contribution of IFN-{gamma} to the V14 NKT cell-mediated allograft acceptance in vivo. These results indicate a critical contribution of V14 NKT cells to the induction of allograft tolerance and provide a useful model to investigate the regulatory role of V14 NKT cells in various immune responses.
