期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2001
卷号：98
期号：5
页码：2781-2785
DOI：10.1073/pnas.031580798
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A study was made of glycine (Gly) and {gamma}-aminobutyric acid (GABA) receptors expressed in Xenopus oocytes injected with rat mRNAs isolated from the encephalon, midbrain, and brainstem of 18-day-old rat embryos. In oocytes injected with encephalon, midbrain, or brainstem mRNAs, the Gly-current amplitudes (membrane current elicited by Gly; 1 mM Gly) were respectively 115 {+/-} 35, 346 {+/-} 28, and 389 {+/-} 22 nA, whereas the GABA-currents (1 mM GABA) were all [≤]40 nA. Moreover, the Gly-currents desensitized faster in oocytes injected with encephalon or brainstem mRNAs. The EC50 for Gly was 611 {+/-} 77 {micro}M for encephalon, 661 {+/-} 28 {micro}M for midbrain, and 506 {+/-} 18 {micro}M for brainstem mRNA-injected oocytes, and the corresponding Hill coefficients were all {approx}2. Strychnine inhibited all of the Gly-currents, with an IC50 of 56 {+/-} 3 nM for encephalon, 97 {+/-} 4 nM for midbrain, and 72 {+/-} 4 nM for brainstem mRNAs. During repetitive Gly applications, the Gly-currents were potentiated by 1.6-fold for encephalon, 2.1-fold for midbrain, and 1.3-fold for brainstem RNA-injected oocytes. Raising the extracellular Ca2+ concentration significantly increased the Gly-currents in oocytes injected with midbrain and brainstem mRNAs. Reverse transcription-PCR studies showed differences in the Gly receptor (GlyR) -subunits expressed, whereas the {beta}-subunit was present in all three types of mRNA. These results indicate differential expression of GlyR mRNAs in the brain areas examined, and these mRNAs lead to the expression of GlyRs that have different properties. The modulation of GlyRs by Ca2+ could play important functions during brain development.
