期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：14
页码：9369-9373
DOI：10.1073/pnas.142298399
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The signaling pathways that lead to the localization of cellular protein to the area of interaction between T cell and antigen-presenting cell and the mechanism by which these molecules are further sorted to the peripheral supramolecular activation cluster or central supramolecular activation cluster regions of the immunologic synapse are poorly understood. In this study, we investigated the functional involvement of CD28 costimulation in the T cell receptor (TCR)-mediated immunologic synapse formation with respect to protein kinase C (PKC){theta} localization. We showed that CD3 crosslinking alone was sufficient to induce PKC{theta} capping in naive CD4+ T cells. Studies with pharmacologic inhibitors and knockout mice showed that the TCR-derived signaling that drives PKC{theta} membrane translocation requires the Src family kinase, Lck, but not Fyn. In addition, a time course study of the persistence of T cell molecules to the immunologic synapse indicated that PKC{theta
