期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：6
页码：3299-3304
DOI：10.1073/pnas.0434590100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Wnt/{beta}-catenin signaling plays key roles in several developmental and pathological processes. Domains of Wnt expression have been extensively investigated in the mouse, but the tissues receiving the signal remain largely unidentified. To define which cells respond to activated {beta}-catenin during mammalian development, we generated the {beta}-catenin-activated transgene driving expression of nuclear {beta}-galactosidase reporter (BAT-gal) transgenic mice, expressing the lacZ gene under the control of {beta}-catenin/T cell factor responsive elements. Reporter gene activity is found in known organizing centers, such as the midhindbrain border and the limb apical ectodermal ridge. Moreover, BAT-gal expression identifies novel sites of Wnt signaling, like notochord, endothelia, and areas of the adult brain, revealing an unsuspected dynamic pattern of {beta}-catenin transcriptional activity. Expression of the transgene was analyzed in mutant backgrounds. In lipoprotein receptor-related protein 6-null homozygous mice, which lack a Wnt coreceptor, BAT-gal staining is absent in mutant tissues, indicating that BAT-gal mice are bona fide in vivo indicators of Wnt/{beta}-catenin signaling. Analyses of BAT-gal expression in the adenomatous polyposis coli (multiple intestinal neoplasia/+) background revealed {beta}catenin transcriptional activity in intestinal adenomas but surprisingly not in normal crypt cells. In summary, BAT-gal mice unveil the entire complexity of Wnt/{beta}-catenin signaling in mammals and have broad application potentials for the identification of Wnt-responsive cell populations in development and disease.
关键词：transgenic mice‖lipoprotein receptor-related protein 6‖adenomatous polyposis coli‖colon cancer
