期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：7
页码：3731-3736
DOI：10.1073/pnas.0637275100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Our originally designed dansylamidoethylcyclen 4 as a biomimetic Zn2+-selective fluorophore has been demonstrated to be a good detector of the apoptosis (induced by an anticancer agent, etoposide, and H2O2) in cancer cells such as HeLa and HL60 cells. The macrocyclic Zn2+ ligand 4 (mostly as a deprotonated form) is cell-permeable to show weak fluorescence (emission at 550 nm), which forms a strong fluorescent 1:1 Zn2+ complex 5 (emission at 530 nm) when Zn2+ is incorporated into the cells by a zinc(II) ionophore pyrithione. Thus formed, Zn2+ complex 5 is cell-impermeable and remains intact over a few hours. When apoptosis in HeLa or HL60 cells is artificially induced, 4 selectively and strongly stains apoptotic cells only at early stages, which was verified by using the conventional apoptosis detection probe annexin V-Cy3. Detection of the apoptotic cells by 4 was perhaps due to significantly increased free Zn2+ flux at early stages of apoptosis. Apoptotic detection by 4 has been compared with a presently available Zn2+ fluorophore, Zinquin 1. We present that 4 has advantages in detection of apoptosis over annexin V-Cy3 and Zinquin 1.
