期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：11
页码：7769-7773
DOI：10.1073/pnas.112225599
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The function of the Rhesus (Rh) complex in the human red cell membrane has been unknown for six decades. Based on the organismal, organ, and tissue distribution of Rh proteins, and on our evidence that their only known paralogues, the ammonium and methylammonium transport proteins (also called methylammonium permeases), are gas channels for NH3, we recently speculated that Rh proteins are biological gas channels for CO2. Like NH3, CO2 differs from other gases in being readily hydrated. We have now tested our speculation by studying expression of the RH1 gene in the photosynthetic microbe Chlamydomonas reinhardtii. Expression of RH1 was high for cells grown in air supplemented with 3% CO2 or shifted from air to high CO2 (3%) for 3 h. Conversely, RH1 expression was low for cells grown in air (0.035% CO2) or shifted from high CO2 to air for 3 h. These results make viable the hypothesis that Rh1 and Rh proteins generally are gas channels for CO2.
