期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：18
页码：11760-11765
DOI：10.1073/pnas.192285999
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The hematopoietic transcription factor GATA-1 regulates erythropoiesis and {beta}-globin expression. Although consensus GATA-1 binding sites exist throughout the murine {beta}-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the {beta}-globin promoters. The hematopoietic factor NF-E2 cooperated with GATA-1 to recruit pol II to the promoters. We propose that only when GATA-1 attracts pol II to the locus control region can pol II access the promoter in a NF-E2-dependent manner.
