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  • 标题:The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain
  • 本地全文:下载
  • 作者:Matthew L. Baker ; Irina I. Serysheva ; Serap Sencer
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2002
  • 卷号:99
  • 期号:19
  • 页码:12155-12160
  • DOI:10.1073/pnas.182058899
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:We used a combination of bioinformatics, electron cryomicroscopy, and biochemical techniques to identify an oxidoreductase-like domain in the skeletal muscle Ca2+ release channel protein (RyR1). The initial prediction was derived from sequence-based fold recognition for the N-terminal region (41-420) of RyR1. The putative domain was computationally localized to the clamp domain in the cytoplasmic region of a 22A structure of RyR1. This localization was subsequently confirmed by difference imaging with a sequence specific antibody. Consistent with the prediction of an oxidoreductase domain, RyR1 binds [3H]NAD+, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor.
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