期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2002
卷号：99
期号：22
页码：14458-14463
DOI：10.1073/pnas.212148999
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Cytoskeleton is believed to contribute to activity-dependent processes underlying neuronal plasticity, such as regulations of cellular morphology and localization of signaling proteins. However, how neuronal activity controls actin cytoskeleton remains obscure. Taking advantage of confocal imaging of enhanced GFP-actin in the primary culture of hippocampal neurons, we show that synaptic activity induces multiple types of actin reorganization, both at the spines and at the somatic periphery. Activation of N-methyl-D-aspartate receptors, accompanied with a local rise in [Ca2+]i, was sufficient to trigger a slow and sustained recruitment of actin into dendritic spines. In contrast, opening of voltage-gated Ca2+ channels rapidly and reversibly enhanced cortical actin at the somatic periphery but not in the spines, in keeping with a high transient rise in somatic [Ca2+]i. These data suggest that spatiotemporal dynamics of [Ca2+]i, triggered by activation of N-methyl-D-aspartate receptors and voltage-gated Ca2+ channels, provides the molecular basis for activity-dependent actin remodeling.
