期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：10
页码：6093-6097
DOI：10.1073/pnas.1031826100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Bovine spongiform encephalopathy contamination of the human food chain most likely resulted from nervous system tissue in mechanically recovered meat used in the manufacture of processed meats. We spiked hot dogs with 263K hamster-adapted scrapie brain (10% wt/wt) to produce an infectivity level of {approx}9 log10 mean lethal doses (LD50) per g of paste homogenate. Aliquots were subjected to short pressure pulses of 690, 1,000, and 1,200 MPa at running temperatures of 121-137{degrees}C. Western blots of PrPres were found to be useful indicators of infectivity levels, which at all tested pressures were significantly reduced as compared with untreated controls: from {approx}103 LD50 per g at 690 MPa to {approx}106 LD50 per g at 1,200 MPa. The application of commercially practical conditions of temperature and pressure could ensure the safety of processed meats from bovine spongiform encephalopathy contamination, and could also be used to study phase transitions of the prion protein from its normal to misfolded state.
关键词：food processing‖scrapie‖bovine spongiform encephalopathy‖ new variant Creutzfeldt-Jakob disease‖prion disease
