期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：12
页码：7389-7394
DOI：10.1073/pnas.1230987100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Precursor protease vesicles are plant-specific compartments containing precursors of enzymes that are thought to participate in the degradation of cellular components in organs undergoing senescence. We report in vivo evidence that the precursor protease vesicle-localized vacuolar processing enzyme-{gamma} (VPE{gamma}) is critical for maturation of the plant vacuolar protease AtCPY. We also provide biochemical and functional evidence that VPE{gamma} is involved in degradation of the vacuolar invertase AtFruct4 in aging tissues. Moreover, a proteomics-based approach identified various proteins found in the vacuoles of aging vpe{gamma} mutants but not in WT plants, suggesting a unique role of VPE{gamma} in protein processing and degradation in Arabidopsis.
