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  • 标题:Murine cytomegalovirus paralyzes macrophages by blocking IFNγ-induced promoter assembly
  • 本地全文:下载
  • 作者:Daniel L. Popkin ; Mark A. Watson ; Elizabeth Karaskov
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2003
  • 卷号:100
  • 期号:24
  • 页码:14309-14314
  • DOI:10.1073/pnas.1835673100
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Macrophages (M{phi}) are activated by IFN{gamma} and are important cellular targets for infection by human and murine cytomegalovirus (MCMV), making it advantageous for CMVs to block IFN{gamma}-induced M{phi} differentiation. We found that MCMV infection inhibited IFN{gamma} regulation of many genes in M{phi}. MCMV infection blocked IFN{gamma} responses at the level of transcription without blocking Janus kinase/signal transducer and activator of transcription pathway activation and targeted IFN response factor 1- and class II transactivator-dependent and independent promoters. MCMV did not alter basal transcription from IFN{gamma}-responsive promoters and left the majority of cellular transcripts unchanged even after 48 h of infection. The effects of MCMV infection were specific to chromosomal rather than transiently transfected promoters. Characterization of the IFN{gamma}-responsive chromosomal class II transactivator promoter revealed that MCMV infection blocked IFN{gamma}-induced promoter assembly, allowing the virus to transcriptionally paralyze infected M{phi} responses while allowing basal transcription to proceed.
  • 关键词:immune evasion ; microarray
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