期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：25
页码：14840-14845
DOI：10.1073/pnas.2335948100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Thermodynamic formulations have been devised to obtain {Delta}G{degrees} values directly from spectroscopic data at a fixed common temperature in nucleic acid duplex-simplex melting curves. In addition, the dependence of melting on salt concentration has been expressed in terms of a stepwise stoichiometric representation, which leads to a specific equation for the partition of the added sodium ions between the different oligonucleotide forms.
