期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2003
卷号：100
期号：26
页码：16006-16011
DOI：10.1073/pnas.2136600100
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Cell surface receptor membrane localization is strongly dependent on protein-protein interactions often involving regulation by phosphorylation/dephosphorylation of the intracellular domains of membrane proteins. The present study was carried out to identify metabotropic glutamate receptor (mGluR) 3 regulatory binding proteins. Using the yeast two-hybrid technique, we found that the 50-aa C-terminal cytoplasmic tail of mGluR3 interacts specifically with protein phosphatase 2C (PP2C). This interaction was confirmed by GST pull-down and coimmunoprecipitation assays. mGluR3 interacts with PP2C, {beta
