期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2004
卷号：101
期号：13
页码：4543-4547
DOI：10.1073/pnas.0400356101
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The peroxisome proliferator-activated receptor {gamma} (PPAR{gamma}) mediates the activity of the insulin-sensitizing thiazolidinediones and plays an important role in adipocyte differentiation and fat accretion. The analysis of PPAR{gamma} functions in mature adipocytes is precluded by lethality of PPAR{gamma}-/- fetuses and tetraploid-rescued pups. Therefore we have selectively ablated PPAR{gamma} in adipocytes of adult mice by using the tamoxifen-dependent Cre-ERT2 recombination system. We show that mature PPAR{gamma}-null white and brown adipocytes die within a few days and are replaced by newly formed PPAR{gamma}-positive adipocytes, demonstrating that PPAR{gamma} is essential for the in vivo survival of mature adipocytes, in addition to its well established requirement for their differentiation. Our data suggest that potent PPAR{gamma} antagonists could be used to acutely reduce obesity.
