期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:2004
卷号:101
期号:15
页码:5488-5493
DOI:10.1073/pnas.0401138101
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:DNA sequencing by synthesis on a solid surface offers new paradigms to overcome limitations of electrophoresis-based sequencing methods. Here we report DNA sequencing by synthesis using photocleavable (PC) fluorescent nucleotides [dUTP-PC-4,4-difluoro-4-bora-3{alpha
关键词:1,3-dipolar azide-alkyne cycloaddition ; DNA chip ; DNA sequencing by synthesis ; 2-nitrobenzyl linker ; photocleavage