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  • 标题:Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
  • 本地全文:下载
  • 作者:Tae Seok Seo ; Xiaopeng Bai ; Hameer Ruparel
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2004
  • 卷号:101
  • 期号:15
  • 页码:5488-5493
  • DOI:10.1073/pnas.0401138101
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:DNA sequencing by synthesis on a solid surface offers new paradigms to overcome limitations of electrophoresis-based sequencing methods. Here we report DNA sequencing by synthesis using photocleavable (PC) fluorescent nucleotides [dUTP-PC-4,4-difluoro-4-bora-3{alpha
  • 关键词:1,3-dipolar azide-alkyne cycloaddition ; DNA chip ; DNA sequencing by synthesis ; 2-nitrobenzyl linker ; photocleavage
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