期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2005
卷号：102
期号：12
页码：4465-4469
DOI：10.1073/pnas.0409839102
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The lysogenic state of bacteriophage {lambda} is exceptionally stable yet the prophage is readily induced in response to DNA damage. This delicate epigenetic switch is believed to be regulated by two proteins; the lysogenic maintenance promoting protein CI and the early lytic protein Cro. First, we confirm, in the native configuration, the previous observation that the DNA loop mediated by oligomerization of CI bound to two distinct operator regions (OL and OR), increases repression of the early lytic promoters and is important for stable maintenance of lysogeny. Second, we show that the presence of the cro gene might be unimportant for the lysogenic to lytic switch during induction of the {lambda} prophage. We revisit the idea that Cro's primary role in induction is instead to mediate weak repression of the early lytic promoters.
