期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2007
卷号：104
期号：40
页码：15829-15834
DOI：10.1073/pnas.0707426104
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：[beta] 1,3-N-acetylglucosaminyltransferase 2 ([beta]3GnT2) is a polylactosamine synthase that synthesizes a backbone structure of carbohydrate structures onto glycoproteins. Here we generated [beta]3GnT2-deficient ([beta]3GnT2-/-) mice and showed that polylactosamine on N-glycans was markedly reduced in their immunological tissues. In WT mice, polylactosamine was present on CD28 and CD19, both known immune costimulatory molecules. However, polylactosamine levels on these molecules were reduced in [beta]3GnT2-/- mice. [beta]3GnT2-/- T cells lacking polylactosamine were more sensitive to the induction of intracellular calcium flux on stimulation with anti-CD3{varepsilon}/CD28 and proliferated more strongly than T cells from WT mice. [beta]3GnT2-/- B cells also showed hyperproliferation on BCR stimulation. Macrophages from [beta]3GnT2-/- mice had higher cell surface CD14 levels and enhanced responses to endotoxin. These results indicate that polylactosamine on N-glycans is a putative immune regulatory factor presumably suppressing excessive responses during immune reactions.
