期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：19
页码：8015-8020
DOI：10.1073/pnas.0903022106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We and others have reported that the vast majority of virus-producing CD4+ T cells during the acute infection of rhesus macaques with simian immunodeficiency virus (SIV) or CXCR4 (X4)-using simian/human immunodeficiency viruses (SHIVs) exhibited a nonactivated phenotype. These findings have been extended to show that resting CD4+ T lymphocytes collected from SIV- or X4-SHIV-infected animals during the first 10 days of infection continue to release virus ex vivo. Furthermore, we observed high frequencies of integrated viral DNA (up to 5.1 x 104 DNA copies per 105 cells) in circulating resting CD4+ T cells during the first 10 days of the infection. Integration of SIV DNA was detected only in memory CD4+ T cells and SHIVs preferentially integrated into resting naive CD4+ T cells. Taken together, these results show that during the acute infection large numbers of resting CD4+ T cells carry integrated nonhuman primate lentiviral DNA and are the major source of progeny virions irrespective of coreceptor usage. Prompt and sustained interventions are therefore required to block the rapid systemic dissemination of virus and prevent an otherwise fatal clinical outcome.
