期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2009
卷号：106
期号：20
页码：8278-8283
DOI：10.1073/pnas.0903390106
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Studying gene expression at different hematopoietic stages provides insights for understanding the genetic basis of hematopoiesis. We analyzed gene expression in human CD34+ hematopoietic cells that represent the stem-progenitor population (CD34+ cells). We collected >459,000 transcript signatures from CD34+ cells, including the de novo-generated 3' ESTs and the existing sequences of full-length cDNAs, ESTs, and serial analysis of gene expression (SAGE) tags, and performed an extensive annotation on this large set of CD34+ transcript sequences. We determined the genes expressed in CD34+ cells, verified the known genes and identified the new genes of different functional categories involved in hematopoiesis, dissected the alternative gene expression including alternative transcription initiation, splicing, and adenylation, identified the antisense and noncoding transcripts, determined the CD34+ cell-specific gene expression signature, and developed the CD34+ cell-transcription map in the human genome. Our study provides a current view on gene expression in human CD34+ cells and reveals that early hematopoiesis is an orchestrated process with the involvement of over half of the human genes distributed in various functions. The data generated from our study provide a comprehensive and uniform resource for studying hematopoiesis and stem cell biology.
