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  • 标题:Nonlinear electrophoretic response yields a unique parameter for separation of biomolecules
  • 本地全文:下载
  • 作者:Joel Pel ; David Broemeling ; Laura Mai
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2009
  • 卷号:106
  • 期号:35
  • 页码:14796-14801
  • DOI:10.1073/pnas.0907402106
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:We demonstrate a unique parameter for biomolecule separation that results from the nonlinear response of long, charged polymers to electrophoretic fields and apply it to extraction and concentration of nucleic acids from samples that perform poorly under conventional methods. Our method is based on superposition of synchronous, time-varying electrophoretic fields, which can generate net drift of charged molecules even when the time-averaged molecule displacement generated by each field individually is zero. Such drift can only occur for molecules, such as DNA, whose motive response to electrophoretic fields is nonlinear. Consequently, we are able to concentrate DNA while rejecting high concentrations of contaminants. We demonstrate one application of this method by extracting DNA from challenging samples originating in the Athabasca oil sands.
  • 关键词:concentration ; DNA ; electrophoresis ; purification ; SCODA
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