期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：12
页码：3282-3286
DOI：10.1073/pnas.70.12.3282
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Heavy and light forms of elongation factor 1 (EF-1) from calf brain have been partially purified. The heterogeneous heavy species (EF-1H) with molecular weights of 2.5 x 105 to over 1 x 106 appears to be a complex or aggregate of the light form of the enzyme (EF-1L); the latter has a molecular weight of between 50,000 and 60,000. EF-1H but not EF-1L, contains significant amounts of free and esterified cholesterol. Although EF-1H and EF-1L are both active in aminoacyl-tRNA binding to ribosomes, EF-1L reacts with GTP and aminoacyl-tRNA more efficiently than EF-1H.
