期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：12
页码：3405-3409
DOI：10.1073/pnas.70.12.3405
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Synthesis of [α] and {beta} chains of hemoglobin was studied in vitro in intact reticulocytes and bone marrow cells. The cells were from rabbits having a variant form of hemoglobin in which L-isoleucine is in the [α] but not in the {beta} chains. This characteristic permitted a selective inhibition of [α]-chain synthesis to be produced by addition to the incubation medium of L-O-methylthreonine, an inhibitor of protein synthesis that is a specific antagonist of L-isoleucine. In studies with reticulocytes, 25 mM L-O-methylthreonine produced a 60-70% inhibition of [α]-chain synthesis, but {beta}-chain synthesis was unaffected even after incubation times for 4 hr. Because reticulocytes contain a pool of uncombined [α] chains which might have obscured the demonstration of an [α] chain-dependent mechanism for {beta}-chain synthesis, subsequent studies were done with bone marrow cells. The latter had little or no detectable [α]-chain pool. A substantial inhibition of [α]-chain synthesis by the bone marrow cells was produced by the isoleucine antagonist but was also accompanied by a significantly decreased rate of {beta}-chain synthesis. These findings suggest that the coordinated synthesis of the complementary [α]- and {beta}-globin chains of hemoglobin may reflect in part a modifying effect of [α]-chain synthesis on the synthesis of {beta} chains.
关键词：globin synthesis ; reticulocytes ; L- O -methylthreonine
