期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1973
卷号：70
期号：12
页码：3806-3809
DOI：10.1073/pnas.70.12.3806
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The 300-MHz nuclear magnetic resonance spectrum of the peptide hormone, oxytocin, in water, was obtained. Extensive nuclear magnetic resonance spindecoupling experiments, including those involving irradiation of the [α]-proton resonance under the water peak, studies using partially deuterated hormone derivatives, and nuclear magnetic resonance studies of some precursor peptides to oxytocin, permitted us to assign most of the proton resonance of the hormone unambiguously. The JN[α] values and temperature dependence of the chemical shift of the peptide amide and carboxamide protons of oxytocin were also obtained. These studies indicate that, in aqueous solution, oxytocin is a flexible molecule possessing several different conformations. Corresponding studies of [4-leucine]oxytocin were also made, with similar results and conclusions.
