期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：10
页码：3892-3895
DOI：10.1073/pnas.71.10.3892
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.
关键词：synthetic DNA ; globin mRNA ; RNA-dependent DNA polymerase ; oligo(dT)-cellulose ; chromatography
