标题：Diagonal Polyacrylamide-Dodecyl Sulfate Gel Electrophoresis for the Identification of Ribosomal Proteins Crosslinked with Methyl-4-Mercaptobutyrimidate
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：10
页码：3946-3950
DOI：10.1073/pnas.71.10.3946
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A diagonal polyacrylamide-dodecyl sulfate gel electrophoresis procedure is described. Its utility is related to the use of the reagent methyl-4-mercaptobutyrimidate as a protein-protein crosslinking reagent. Crosslinking with this reagent occurs through the formation of intermolecular disulfide bonds. Oxidized proteins are separated in one dimension by electrophoresis under non-reducing conditions and in the second dimension under reducing conditions. All proteins except those derived from crosslinked species fall on a diagonal. Methods are described for the identification of the separated monomeric components of crosslinked species. The technique has been applied to the 30S ribosomal subunit of Escherichia coli, and the new crosslinked dimer, S4-S13, has been identified.
