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  • 标题:Location of the Spike Glycoproteins in the Semliki Forest Virus Membrane
  • 本地全文:下载
  • 作者:Henrik Garoff ; Kai Simons
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1974
  • 卷号:71
  • 期号:10
  • 页码:3988-3992
  • DOI:10.1073/pnas.71.10.3988
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Labeling experiments with formyl-[35S]-methionyl sulfone methylphosphate and crosslinking studies with dimethylsuberimidate suggest that the spike glycoproteins of Semliki Forest virus extend through the viral membrane into close contact with the nucleocapsid. Based on this finding, we present a mechanism for the formation of virus-specific patches in the host cell plasma membrane during virus assembly.
  • 关键词:formyl-[35S]methionyl sulfone methylphosphate ; dimethylsuberimidate ; peptide mapping ; sodium dodecyl sulfate-gel electrophoresis
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