期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：10
页码：4120-4124
DOI：10.1073/pnas.71.10.4120
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The E. coli proteins that catalyze the conversion of {phi}X174 single-stranded DNA to duplex DNA have now been purified extensively. The reaction depends on dnaB, dnaC(D), dnaE, and dnaG gene products, DNA elongation factors I and II, E. coli DNA binding protein, and two additional E. coli proteins, replication factors X and Y. DNA synthesis by these proteins requires {phi}X174 viral DNA, dNTPs, Mg+2, and ATP. The product synthesized is full-length linear {phi}X174 DNA. The reaction has been resolved into two steps. The first step involves the interaction of ATP and {phi}X174 DNA with dnaB and dnaC(D) gene products, E. coli DNA binding protein, and replication factors X and Y in the absence of dNTPs. Subsequent dNMP incorporation requires the addition of DNA polymerase III, DNA elongation factors I and II, dnaG gene product, and dNTPs.
关键词：E. coli dna gene products ; E. coli DNA binding protein ; DNA elongation factors I and II ; replication factor X and factor Y ; DNA polymerase III
