期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：10
页码：4241-4245
DOI：10.1073/pnas.71.10.4241
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Soluble extracts of mouse cells with Fv-1n or Fv-1b gene alleles specifically and reciprocally inhibit infection of B- or N-tropic mouse leukemia viruses in permissive cell cultures. NB-tropic virus infection was not inhibited by either cell extract. Extracts from Fv-1- cells did not inhibit infection by the three virus host-range types, but N- or B-tropic virus infection of Fv-1- cells was inhibited by extracts of the nonpermissive cells, and Fv-1nb cell extracts inhibited both viruses. The maximum degree of inhibition was 50-80% as determined by immunofluorescent or plaque assays, with extracts containing up to 500 {micro}g/ml of nonpermissive cell protein. The inhibitor(s) is relatively unstable since activity is lost after 2 hr at 37{degrees} or 30 min at 56{degrees}. The inhibitor(s) was most effective if added 2 hr before or within 2 hr after infection, did not react with the virus directly, inhibit virus attachment, or inhibit the normal cell functions tested. These results indicate that nonpermissive mouse cells contain a product, possibly determined by the Fv-1 gene, which inhibits some early postpenetration event(s) in leukemia virus infection.
