期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：5
页码：1710-1714
DOI：10.1073/pnas.71.5.1710
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Chicks were injected intra-cerebrally with [3H]leucine, and 3H-labeled microtubules were assembled in vitro in the brain supernatants. Pieces of these 3H-labeled microtubules were then incubated with unlabeled brain tubulin subunits under conditions where the subunits assembled onto the labeled pieces. Electron microscopic autoradiography of the negatively-stained microtubules showed all of the radioactivity at one end of the tubules as they increased in length. This clearly demonstrated that the microtubules of brain in this in vitro system were being assembled unidirectionally.
关键词：autoradiography ; electron microscopy ; viscometry
