期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：5
页码：1969-1973
DOI：10.1073/pnas.71.5.1969
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A portion of the DNA synthesized by HEp.2 cells after short incubation in the presence of BrdU bands in a CsCl gradient at a distinct peak of density 1.715-1.718 g/cm3. Inhibition of DNA synthesis by methyl methanesulfonate results in an increased proportion of this "intermediate density" DNA. Cells labeled and subsequently chased in nonradioactive BrdU yield only hybrid DNA. Treatment with single-strand-specific deoxyribonuclease converts a portion of the intermediate density material to molecules of hybrid density (1.755 g/cm3). The data suggest an intermediate in DNA replication that contains a single-stranded region in the parental strand and that accumulates when DNA synthesis is blocked by an alkylation-induced lesion.
关键词：alkylating agents ; replication ; single-stranded DNA
