期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：5
页码：1758-1762
DOI：10.1073/pnas.71.5.1758
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have developed a cell-free system to study bacteriophage {lambda} DNA replication. Maximal DNA synthesis in vitro requires the four deoxynucleoside triphosphates, ATP, and exogenous {lambda} DNA. DNA synthesis requires the products of the phage O and P genes but is not inhibited by {lambda} repressor. The kinetics of synthesis is linear for 10-15 min; however, the product of synthesis amounts to only 0.5-1% of the added template DNA. As judged by isopycnic analysis, extensive regions of the template are copied. Sedimentation analysis indicates that all of the product consists of short (11S) DNA chains. Fractions partially purified from {lambda}O+P+-infected cell extracts will complement extracts prepared from {lambda}O- or {lambda}P--infected cells.
关键词：Escherichia coli ; DNA initiation ; DNA polymerase
