期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：5
页码：2022-2026
DOI：10.1073/pnas.71.5.2022
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Endonuclease II (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.30 ) of Escherichia coli has been shown to break phosphodiester bonds in alkylated DNA and depurinated DNA. The hypothesis that depurination is a step in the mechanism of the reaction with alkylated DNA is supported by in vitro experiments with DNA reacted with N-methyl-N-nitrosourea. Endonuclease II releases O6-methylguanine and 3-methyladenine, but not 7-methylguanine, from DNA that has been methylated by the carcinogen N-methyl-N-nitrosourea.
关键词：DNA repair ; methylnitrosourea ; endonuclease II ; chemical carcinogenesis
