期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1974
卷号：71
期号：5
页码：1995-1998
DOI：10.1073/pnas.71.5.1995
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The complete amino-acid sequence was determined for a rabbit-antibody light chain of allotype b4. The light chain was recovered from approximately 20 g of homogeneous antibody to group C streptococcal carbohydrate, which was obtained from an immunized rabbit. Several enzymic digestions were used to obtain the overlapping peptides necessary for the sequence determination. The L chain has 210 amino-acid residues, as determined by sequence analysis, and a molecular mass of 23,060 daltons. This antibody light chain contains seven half-cystine residues characteristic of the {kappa}B light-chain class. The amino-terminal sequence was determined to be Ala-Asp-Ile-Val-Met, indicating that the chain belongs to the V{kappa}I subgroup.
