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  • 标题:Characterization of a novel, low-molecular-weight DNA-binding protein from Escherichia coli
  • 本地全文:下载
  • 作者:J Rouvière-Yaniv ; F Gros
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1975
  • 卷号:72
  • 期号:9
  • 页码:3428-3432
  • DOI:10.1073/pnas.72.9.3428
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A low-molecular-weight (7000), heat-stable protein--HU--that stimulates transcription of bacteriophage lambda DNA by E. coli RNA polymerase was purified from E. coli extracts using affinity chromatography on DNA-cellulose. HU binds to native DNA, resulting in an apparent thickening of the DNA chains as revealed by electron microscopy. Contrary to DNA unwinding proteins, it causes no destabilization of the double helix. HU differs from previously described transcription factors (H1, D, etc.) and from the low-molecular-weight omega subunit of the RNA polymerase. By its amino-acid composition and characteristics, HU displays an interesting resemblance to some eukaryotic histones, such as H2B and H1.
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