首页    期刊浏览 2024年12月01日 星期日
登录注册

文章基本信息

  • 标题:Histones H3 and H4 interact with the ends of nucleosome DNA
  • 本地全文:下载
  • 作者:R T Simpson
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1976
  • 卷号:73
  • 期号:12
  • 页码:4400-4404
  • DOI:10.1073/pnas.73.12.4400
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Isolated HeLa cell nucleosomes (core particles) were labeled at the 5'-termini of their DNA with 32P using [gamma-32P]ATP and polynucleotide kne by sequential methylation, depurination, Schiff base formation, and reduction with sodium borhydride. After digestion of the noncrosslinked DNA by DNase I and venom phosphodiesterase, histones were separated by gel electrophoresis and those crosslinked to the 5'-termini were identified by 32P-autoradiography. Histones H3 and H4 occur with equeal frequency as the nearest protein neighbors to the end of the DNA in nucleosomes. Histone arrangements within the core particle compatible with these results are discussed.
国家哲学社会科学文献中心版权所有