期刊名称:Proceedings of the National Academy of Sciences
印刷版ISSN:0027-8424
电子版ISSN:1091-6490
出版年度:1976
卷号:73
期号:12
页码:4420-4423
DOI:10.1073/pnas.73.12.4420
语种:English
出版社:The National Academy of Sciences of the United States of America
摘要:Collagen produced by chick neural retina tissue in vitro has the properties of type II collagen. Isolated neural retina tissue from stage 29-32 chick embryos incorporated [14C]glycine and [3H]proline into collagen consisting of alpha1 chains and a higher molecular weight species. The peptides produced by cyanogen bromide digestion of the collagen are identical to those from authentic type II collagen from cartilage in charge properties, as determined by carboxymethyl-cellulose chromatography, and size distribution, as determined by sodium dodecyl sulfate-acrylamide gel electrophoresis.