期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：12
页码：4555-4559
DOI：10.1073/pnas.73.12.4555
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Extracts of serum-free medium, conditioned by contact with SV40-transformed BHK cells, stimulate DNA synthesis in cultured fibroblasts. From this source, we have purified a homogenous basic protein of 18,000 molecular weight, termed fibroblast derived growth factor. In submicrogram quantities, fibroblast derived growth factor stimulates DNA synthesis in mouse 3T3 cells, in the absence of added serum. Prior to the onset of DNA synthesis, both serum and fibroblast derived growth factor induce an array of nearly simultaneous biochemical changes in the membrane to 3T3 cells that include stimulation of the uptake of nucleosides, 2-deoxyglucose, and 86Rb+. These results strongly suggest that the early events are integral components of the proliferative response, rather than coincidental effects of nonmitogenic molecules present in serum.
