期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1976
卷号：73
期号：12
页码：4560-4564
DOI：10.1073/pnas.73.12.4560
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A comparative study of polypeptide composition and freeze-fracture morphology of chloroplast membranes isolated from different, but photosynthetically active, regions of a romaine lettuce shoot is presented. Chloroplasts prepared from outer dark-green leaves possess a fully developed light-harvesting chlorophyll-protein complex, have low chlorophyll a:b ratios and display fracture faces similar to those found in other higher plant chloroplast membranes; chloroplasts from leaves more to the interior of the shoot, have a much lower content of chlorophyll, show high chlorophyll a:b ratios, are depleted in components of the light-harvesting chlorophyll-protein complex, and exhibit extensive modifications of their fracture faces. We have also re-examined the freeze-fracture morphology of chloroplast membranes from a barley b-deficient mutant that lacks the light-harvesting chlorophyll-protein complex. A tentative interpretation of our findings suggests correlating the assembly of the light-harvesting chlorophyll-protein complex into the chloroplast membranes with the appearance of large freeze-fracture B face particles in the stacked interior-membrane region, and differentiation of typical fracture faces.
