标题：Segregation at a locus determining an immunoglobulin genetic marker for the light chain variable region affects inheritance of expression of an idiotype
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：10
页码：4600-4604
DOI：10.1073/pnas.74.10.4600
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Previous investigations have demonstrated close genetic linkage between loci governing expression of strain-specific idiotypes and immunoglobulin heavy (H) chain allotype (i.e., the CH locus). This linkage is presumed to reflect polymorphism of VH genes (or of their expression) linked to the polymorphic CH locus. That there was no apparent involvement of light (L) chain loci (thought to be unlinked to H chain) in inheritance of the idiotype-positive (Id+) phenotype was surprising, because the L chain is required for formation of each of the idiotypes studied at the chemical level. However, previous studies involving backcrosses of F1(Id+ x Id-) mice to the Id- parental strain have never employed as the Id- parent one of several inbred strains shown by G. M. Edelman and P. D. Gottlieb [(1970) Proc. Natl. Acad. Sci. USA 67, 1191-1199] to express a VL-region polymorphism. Among backcrosses performed in the present study, one involved the A/J strain as the Id+ parent and the PL/J strain, one of the several strains with and L chain polymorphism, as the Id- parent. Whereas in three other backcrosses performed, idiotype expression segregated with H chain allotype, in the backcross to PL/J, all mice producing the characteristic A/J Id+ phenotype were A/J allotype+, but not all A/J allotype+ mice were Id+. Typing of backcross progeny for the Ly-3 thymocyte alloantigens, controlled by a locus closely linked to that governing the L chain polymorphism (called the VK-1 locus), indicated that only the Ly-3 heterozygotes expressed the characteristic A/J Id+ phenotype. Thus, all Id+ backcross mice inherited both the H chain allotype and the Ly-3 locus (and closely linked L chain-related locus) from the Id+ A/J strain. This suggests that, when strains with L chain polymorphisms are included in genetic studies of idiotype expression, segregation of loci governing L chain expression may be found to contribute to inheritance of the Id+ character. In addition, these studies may be taken as further evidence that the VL-region repertoire of the PL/J strain may differ considerably from that of most other inbred strains of mice.
关键词：idiotype inheritance ; V and C genes ; light chain allotypes ; allotype-idiotype linkage ; VK-1 locus
