期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1977
卷号：74
期号：8
页码：3184-3188
DOI：10.1073/pnas.74.8.3184
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Dimethyl sulfoxide-induced Friend cells were labeled for periods of 5-60 min. The denatured RNA was fractionated by sucrose gradient centrifugation and the distribution of - and {beta}-globin-specific [3H]RNA was determined by hybridization to hybrid plasmids containing mouse - and {beta}-globin DNA, respectively. After 5 min of labeling, a 15S peak of {beta}-globin-specific (but not -globin-specific) [3H]RNA was detected, next to an equal amount of 10S {beta}-globin [3H]RNA. With increasing periods of labeling, the amount of 15S {beta}-globin [3H]RNA remained constant but the amount 10S {beta}-globin [3H]RNA increased steadily. -Globin-specific [3H]RNA sedimented at 11 S after 5 min of labeling and at 9.5 S after longer labeling periods. Analysis of 15S globin-specific [3H]RNA purified by the poly(dC)-cDNA method [Curtis, P. J. & Weissmann, C. (1976) J. Mol. Biol. 106, 1061-1075] showed oligonucleotides characteristic of {beta}-globin mRNA but not of -globin mRNA, as well as about 20 new oligonucleotides. Our results suggest that 10S {beta}-globin mRNA arises via a 15S precursor that has a half-life of 5 min or less; 9.5S -globin mRNA may be derived from an 11S precursor.
