期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：1
页码：266-269
DOI：10.1073/pnas.75.1.266
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The involvement of long chain aldehyde in bacterial luminescence was known both from its being required for light emission in the in vitro reaction with pure luciferase and from its ability to stimulate luminescence in vivo in a certain class of dark "aldehyde" mutants. We have found that the luminescence of some (but not all) of such aldehyde mutants is also stimulated by long chain aliphatic fatty acids, with a marked specificity for myristic (tetradecanoic) acid. This stimulation has been demonstrated in aldehyde mutants of two species of luminous bacteria, Beneckea harveyi and Photobacterium fischeri. The responses, both in intensity and yield, are proportional to the amount of added tetradecanoic acid over a 1000-fold range, down to 10 pmol ml-1. Unsaturated long chain fatty acids are potent inhibitors of the tetradecanoic acid stimulation, but they do not effect the in vivo luminescence of wild-type bacteria.
