首页    期刊浏览 2025年06月11日 星期三
登录注册

文章基本信息

  • 标题:Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker
  • 本地全文:下载
  • 作者:T R Skopek ; H L Liber ; J J Krolewski
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1978
  • 卷号:75
  • 期号:1
  • 页码:410-414
  • DOI:10.1073/pnas.75.1.410
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:We have developed a quantitative forward mutation assay using Salmonella typhimurium, in which resistance to the purine analog 8-azaguanine is used as a genetic marker. We present the assay protocol, the concentration-dependent toxicity and mutagenicity of five known mutagens (N-methyl-N'-nitro-N-nitrosoguanidine, ICR-191, 9-aminoacridine, dimethylnitrosamine, and benzo[a]pyrene), and reconstruction experiments testing the assay for possible bias. The relative merits of forward versus reverse mutation assays are discussed.
国家哲学社会科学文献中心版权所有