期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：11
页码：5462-5465
DOI：10.1073/pnas.75.11.5462
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A nitroxide spin-label probe was attached directly to a propionic acid group of heme in either the or the {beta} chain of hemoglobin. The electron paramagnetic resonance (EPR) spectrum of the spin label is altered by the spin-state change of the heme iron to which the spin label is attached. These hybrid hemoglobins showed normal optical and functional properties, indicating that the attachment of the spin label did not perturb the function of hemoglobin. Upon deoxygenation of -heme-spin-labeled hemoglobin, EPR signals changed proportionally with oxygen saturation (determined by measuring absorption spectra). This result indicates that there is no binding preference between the and {beta} chains of hemoglobin. However, the cross plot for the fraction of the EPR changes vs. the fraction of oxygen saturation deviated significantly from the diagonal straight line in response to the addition of 2,3-diphosphoglycerate and inositol hexaphosphate. The deviation indicated that the EPR change precedes the optical change at low oxygen tension. This result implies that, in the presence of organic phosphate, oxygen binds preferentially to the subunit of deoxyhemoglobin. This conclusion was supported by the result obtained with {beta}-heme-spin-labeled hemoglobin: the direction of the deviation for {beta}-heme-spin-labeled hemoglobin in the presence of diphosphoglycerate and inositol hexaphosphate was opposite to that obtained for -heme-spin-labeled hemoglobin. However, the curve deviated even in the absence of organic phosphate. This deviation for {beta}-heme-spin-labeled hemoglobin can be explained by the intersubunit interaction of hemoglobin. From these results, it was concluded that, in the absence of organic phosphate, oxygen combines with the and {beta} chains with equal probability whereas, in the presence of organic phosphate, oxygen binds preferentially to the chains of hemoglobin.
