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  • 标题:DNA cloning in Bacillus subtilis
  • 本地全文:下载
  • 作者:S D Ehrlich
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1978
  • 卷号:75
  • 期号:3
  • 页码:1433-1436
  • DOI:10.1073/pnas.75.3.1433
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:A plasmid pC194, encoding resistance to chloramphenicol, can serve as a cloning vector in Bacillus subtilis 168 for other HindIII-cleaved DNA segments. Replicons constructed by linking pC194 to several Escherichia coli plasmids can be used to introduce and compare the expression of the same genes in these two bacterial hosts.
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