期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1978
卷号：75
期号：9
页码：4484-4488
DOI：10.1073/pnas.75.9.4484
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Lymphocytes were assessed for the presence of surface actin and myosin by lactoperoxidase-catalyzed iodination and indirect immunofluorescence using antisera against purified pig skeletal muscle actin and pig smooth muscle myosin. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis of 125I-labeled pig, mouse, and human B lymphocytes revealed an intense radioactive band of 43,000 molecular weight, whereas pig and mouse T lymphocytes gave a much less intense band. This band comigrated with actin, was nonglycosylated as judged by lack of binding to lentil lectin-Sepharose, was bound specifically by myosin fibers, and could be distinguished from a polypeptide of similar mobility derived from the major histocompatibility antigens. These results suggest that actin is present on the surface of B lymphocytes and, to a lesser extent, on T lymphocytes. Pig, mouse, and human Ig-bearing cells were stained by antiactin and antimyosin antisera, as judged by indirect immunofluorescence, whereas non-Ig-bearing cells were not stained. Antibody binding, however, was depleted by adsorbing the antisera with Ig-Sepharose. It was concluded that the immunofluorescence results are misleading and reflect the presence of antibodies that crossreact with Ig.
