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  • 标题:Expression in Escherichia coli of chemically synthesized genes for human insulin
  • 本地全文:下载
  • 作者:D V Goeddel ; D G Kleid ; F Bolivar
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1979
  • 卷号:76
  • 期号:1
  • 页码:106-110
  • DOI:10.1073/pnas.76.1.106
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. The insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. Complete purification of the A chain and partial purification of the B chain were achieved. These products were mixed, reduced, and reoxidized. The presence of insulin was detected by radioimmunoassay.
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