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  • 标题:Absence of filipin-sterol complexes from large coated pits on the surface of culture cells
  • 本地全文:下载
  • 作者:R Montesano ; A Perrelet ; P Vassalli
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:1979
  • 卷号:76
  • 期号:12
  • 页码:6391-6395
  • DOI:10.1073/pnas.76.12.6391
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Monolayer cultures of normal or transformed fibroblasts and of liver cells fixed in a glutaraldehyde solution containing 300 microM filipin, a sterol-specific polyene antibiotic, were freeze-fractured to study the distribution of cholesterol within their plasma membranes. Filipin-sterol complexes, recognizable as 25- to 30-nm protuberances scattered in the fracture face of plasma membrane, were absent from invaginations corresponding to large, bristle-coated pits (and possibly also from small, flask-shaped invaginations). These results suggest that invaginating regions on the cell surface are specialized plasma membrane domains with a lower cholesterol content than the surrounding membrane. The localized change in membrane fluidity due to the low cholesterol concentration could play a role in endocytosis.
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