期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1979
卷号：76
期号：12
页码：6351-6355
DOI：10.1073/pnas.76.12.6351
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Planar lipid bilayers containing reaction centers from Rhodopseudomonas sphaeroides R-26 were formed by apposing two reaction center--lipid monolayers formed from a reaction center--lipid complex in hexane. Secondary donors (cytochrome c) and acceptors (ubiquinone-0) were added on opposite sides of the membrane. Upon illumination, this system generated transient and steady-state voltages and currents. The wavelength dependence of the photoresponse matched the absorption spectrum of reaction centers. A simple model based on the transfer of charges across the membrane that explains the salient features of the photoresponse is presented.
关键词：photosynthesis ; membrane reconstitution ; chemosmotic energy coupling ; transmembrane electron transfer
